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biotinylated vegetable lectins  (Vector Laboratories)


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    Vector Laboratories biotinylated vegetable lectins
    Binding of K88ad adhesin to neutral glycosphingolipids from adhesive and nonadhesive phenotypes of pigs. Neutral glycosphingolipids (100 μg) from five phenotypes of pigs (A [lane 2], E [lane 3], B [lane 4], C [lane 5], and D [lane 6]) were separated on HPTLC plates as described in Materials and Methods. Chromatograms were either stained with orcinol-sulfuric acid reagent (panel I) or incubated with <t>biotinylated</t> K88ad adhesin (panel II) as described in Materials and Methods. Lane 1 contains glycolipid standards Lc2Cer (2), Gb4Cer (4), and Gb5Cer (5). The arrowheads indicate the positions of IGLad.
    Biotinylated Vegetable Lectins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated vegetable lectins/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    biotinylated vegetable lectins - by Bioz Stars, 2026-02
    86/100 stars

    Images

    1) Product Images from "Identification of an Intestinal Neutral Glycosphingolipid as a Phenotype-Specific Receptor for the K88ad Fimbrial Adhesin of Escherichia coli † "

    Article Title: Identification of an Intestinal Neutral Glycosphingolipid as a Phenotype-Specific Receptor for the K88ad Fimbrial Adhesin of Escherichia coli †

    Journal:

    doi:

    Binding of K88ad adhesin to neutral glycosphingolipids from adhesive and nonadhesive phenotypes of pigs. Neutral glycosphingolipids (100 μg) from five phenotypes of pigs (A [lane 2], E [lane 3], B [lane 4], C [lane 5], and D [lane 6]) were separated on HPTLC plates as described in Materials and Methods. Chromatograms were either stained with orcinol-sulfuric acid reagent (panel I) or incubated with biotinylated K88ad adhesin (panel II) as described in Materials and Methods. Lane 1 contains glycolipid standards Lc2Cer (2), Gb4Cer (4), and Gb5Cer (5). The arrowheads indicate the positions of IGLad.
    Figure Legend Snippet: Binding of K88ad adhesin to neutral glycosphingolipids from adhesive and nonadhesive phenotypes of pigs. Neutral glycosphingolipids (100 μg) from five phenotypes of pigs (A [lane 2], E [lane 3], B [lane 4], C [lane 5], and D [lane 6]) were separated on HPTLC plates as described in Materials and Methods. Chromatograms were either stained with orcinol-sulfuric acid reagent (panel I) or incubated with biotinylated K88ad adhesin (panel II) as described in Materials and Methods. Lane 1 contains glycolipid standards Lc2Cer (2), Gb4Cer (4), and Gb5Cer (5). The arrowheads indicate the positions of IGLad.

    Techniques Used: Binding Assay, High Performance Thin Layer Chromatography, Staining, Incubation

    Determination of the binding of selected biotinylated lectins
    Figure Legend Snippet: Determination of the binding of selected biotinylated lectins

    Techniques Used: Binding Assay

    Binding of K88ad adhesin to intestinal neutral glycosphingolipids after β-galactosidase treatment. Neutral glycosphingolipids from a phenotype A animal were treated with β-galactosidase as described in Materials and Methods. Both treated (lane 3) and untreated (lane 2) glycosphingolipids (100 μg) were separated on HPTLC plates as described in Materials and Methods. Chromatograms were stained with orcinol-sulfuric acid reagent (A), or incubated with biotinylated K88ad adhesin (B) or RCA120 (C) as described in Materials and Methods. Lane 1 contains glycolipid standards Lc2Cer (2), Gb4Cer (4) and Gb5Cer (5). The arrowheads indicate the positions of IGLad.
    Figure Legend Snippet: Binding of K88ad adhesin to intestinal neutral glycosphingolipids after β-galactosidase treatment. Neutral glycosphingolipids from a phenotype A animal were treated with β-galactosidase as described in Materials and Methods. Both treated (lane 3) and untreated (lane 2) glycosphingolipids (100 μg) were separated on HPTLC plates as described in Materials and Methods. Chromatograms were stained with orcinol-sulfuric acid reagent (A), or incubated with biotinylated K88ad adhesin (B) or RCA120 (C) as described in Materials and Methods. Lane 1 contains glycolipid standards Lc2Cer (2), Gb4Cer (4) and Gb5Cer (5). The arrowheads indicate the positions of IGLad.

    Techniques Used: Binding Assay, High Performance Thin Layer Chromatography, Staining, Incubation

    Binding of K88ad adhesin to intestinal neutral glycosphingolipids after α-fucosidase treatment. Neutral glycosphingolipids from a phenotype A animal were treated with α-fucosidase as described in Materials and Methods. Both treated (lane 3) and untreated (lane 2) glycosphingolipids (100 μg) were separated on HPTLC plates as described in Materials and Methods. Chromatograms were stained with orcinol-sulfuric acid reagent (A) or incubated with biotinylated K88ad adhesin (B) or UEA (C) as described in Materials and Methods. Lane 1 contains glycolipid standards Lc2Cer (2), Gb4Cer (4) and Gb5Cer (5). The arrowheads indicate the positions of IGLad.
    Figure Legend Snippet: Binding of K88ad adhesin to intestinal neutral glycosphingolipids after α-fucosidase treatment. Neutral glycosphingolipids from a phenotype A animal were treated with α-fucosidase as described in Materials and Methods. Both treated (lane 3) and untreated (lane 2) glycosphingolipids (100 μg) were separated on HPTLC plates as described in Materials and Methods. Chromatograms were stained with orcinol-sulfuric acid reagent (A) or incubated with biotinylated K88ad adhesin (B) or UEA (C) as described in Materials and Methods. Lane 1 contains glycolipid standards Lc2Cer (2), Gb4Cer (4) and Gb5Cer (5). The arrowheads indicate the positions of IGLad.

    Techniques Used: Binding Assay, High Performance Thin Layer Chromatography, Staining, Incubation

    Binding of K88ad adhesin to purified neutral glycosphingolipids. Two micrograms of Lc3Cer (lane 1), Lc3Cer plus Lc4Cer (lane 2), nLc4Cer (lane 3), III4FucLc4Cer or Lea (lane 4), III3FucnLc4Cer or Lex (lane 5), V3FucnLc6Cer (lane 6), and VI2FucnLc6Cer (lane 7) were separated on HPTLC. These glycosphingolipids were either stained with orcinol-sulfuric acid reagent (A) or incubated with biotinylated K88ad adhesin (B) as described in Materials and Methods.
    Figure Legend Snippet: Binding of K88ad adhesin to purified neutral glycosphingolipids. Two micrograms of Lc3Cer (lane 1), Lc3Cer plus Lc4Cer (lane 2), nLc4Cer (lane 3), III4FucLc4Cer or Lea (lane 4), III3FucnLc4Cer or Lex (lane 5), V3FucnLc6Cer (lane 6), and VI2FucnLc6Cer (lane 7) were separated on HPTLC. These glycosphingolipids were either stained with orcinol-sulfuric acid reagent (A) or incubated with biotinylated K88ad adhesin (B) as described in Materials and Methods.

    Techniques Used: Binding Assay, Purification, High Performance Thin Layer Chromatography, Staining, Incubation

    Comigration of IGLad and nLc4Cer on HPTLC plates. Neutral glycosphingolipids from a phenotype A animal (100 μg, lane 2), and nLc4Cer (2 μg, lane 3) were separated on HPTLC. These glycosphingolipids were either stained with orcinol-sulfuric acid reagent (A) or incubated with biotinylated K88ad adhesin (B) as described in Materials and Methods. Lane 1 contains glycolipid standards (Lc2Cer [2], Gb4Cer [4], and Gb5Cer [5]). The arrowheads indicate the position of IGLad.
    Figure Legend Snippet: Comigration of IGLad and nLc4Cer on HPTLC plates. Neutral glycosphingolipids from a phenotype A animal (100 μg, lane 2), and nLc4Cer (2 μg, lane 3) were separated on HPTLC. These glycosphingolipids were either stained with orcinol-sulfuric acid reagent (A) or incubated with biotinylated K88ad adhesin (B) as described in Materials and Methods. Lane 1 contains glycolipid standards (Lc2Cer [2], Gb4Cer [4], and Gb5Cer [5]). The arrowheads indicate the position of IGLad.

    Techniques Used: High Performance Thin Layer Chromatography, Staining, Incubation



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    biotinylated vegetable lectins  (Vector Laboratories)
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    Vector Laboratories biotinylated vegetable lectins
    Binding of K88ad adhesin to neutral glycosphingolipids from adhesive and nonadhesive phenotypes of pigs. Neutral glycosphingolipids (100 μg) from five phenotypes of pigs (A [lane 2], E [lane 3], B [lane 4], C [lane 5], and D [lane 6]) were separated on HPTLC plates as described in Materials and Methods. Chromatograms were either stained with orcinol-sulfuric acid reagent (panel I) or incubated with <t>biotinylated</t> K88ad adhesin (panel II) as described in Materials and Methods. Lane 1 contains glycolipid standards Lc2Cer (2), Gb4Cer (4), and Gb5Cer (5). The arrowheads indicate the positions of IGLad.
    Biotinylated Vegetable Lectins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated vegetable lectins/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    biotinylated vegetable lectins - by Bioz Stars, 2026-02
    86/100 stars
    		  Buy from Supplier

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    Binding of K88ad adhesin to neutral glycosphingolipids from adhesive and nonadhesive phenotypes of pigs. Neutral glycosphingolipids (100 μg) from five phenotypes of pigs (A [lane 2], E [lane 3], B [lane 4], C [lane 5], and D [lane 6]) were separated on HPTLC plates as described in Materials and Methods. Chromatograms were either stained with orcinol-sulfuric acid reagent (panel I) or incubated with biotinylated K88ad adhesin (panel II) as described in Materials and Methods. Lane 1 contains glycolipid standards Lc2Cer (2), Gb4Cer (4), and Gb5Cer (5). The arrowheads indicate the positions of IGLad.

    Journal:

    Article Title: Identification of an Intestinal Neutral Glycosphingolipid as a Phenotype-Specific Receptor for the K88ad Fimbrial Adhesin of Escherichia coli †

    doi:

    Figure Lengend Snippet: Binding of K88ad adhesin to neutral glycosphingolipids from adhesive and nonadhesive phenotypes of pigs. Neutral glycosphingolipids (100 μg) from five phenotypes of pigs (A [lane 2], E [lane 3], B [lane 4], C [lane 5], and D [lane 6]) were separated on HPTLC plates as described in Materials and Methods. Chromatograms were either stained with orcinol-sulfuric acid reagent (panel I) or incubated with biotinylated K88ad adhesin (panel II) as described in Materials and Methods. Lane 1 contains glycolipid standards Lc2Cer (2), Gb4Cer (4), and Gb5Cer (5). The arrowheads indicate the positions of IGLad.

    Article Snippet: Biotinylated vegetable lectins (concanavalin A lectin [ConA], soybean agglutinin [SBA], peanut agglutinin [PNA], Sambucus nigra agglutinin [SNA], Bandeira simplicifolia lectin [BSL II], Datura stramonium lectin [DSL], Jacalin, wheat germ agglutinin [WGA], Ulex europaeus agglutinin [UEA], Ricinus communis agglutinin [RCA 120 ], Maackia amurensis lectin I and II [MAL I and MAL II], Erythrina cristagalli lectin [ECL], Vicia villosa agglutinin [VVA]) were obtained from Vector Laboratories, Inc. (Burlingame, Calif.).

    Techniques: Binding Assay, High Performance Thin Layer Chromatography, Staining, Incubation

    Determination of the binding of selected biotinylated lectins

    Journal:

    Article Title: Identification of an Intestinal Neutral Glycosphingolipid as a Phenotype-Specific Receptor for the K88ad Fimbrial Adhesin of Escherichia coli †

    doi:

    Figure Lengend Snippet: Determination of the binding of selected biotinylated lectins

    Article Snippet: Biotinylated vegetable lectins (concanavalin A lectin [ConA], soybean agglutinin [SBA], peanut agglutinin [PNA], Sambucus nigra agglutinin [SNA], Bandeira simplicifolia lectin [BSL II], Datura stramonium lectin [DSL], Jacalin, wheat germ agglutinin [WGA], Ulex europaeus agglutinin [UEA], Ricinus communis agglutinin [RCA 120 ], Maackia amurensis lectin I and II [MAL I and MAL II], Erythrina cristagalli lectin [ECL], Vicia villosa agglutinin [VVA]) were obtained from Vector Laboratories, Inc. (Burlingame, Calif.).

    Techniques: Binding Assay

    Binding of K88ad adhesin to intestinal neutral glycosphingolipids after β-galactosidase treatment. Neutral glycosphingolipids from a phenotype A animal were treated with β-galactosidase as described in Materials and Methods. Both treated (lane 3) and untreated (lane 2) glycosphingolipids (100 μg) were separated on HPTLC plates as described in Materials and Methods. Chromatograms were stained with orcinol-sulfuric acid reagent (A), or incubated with biotinylated K88ad adhesin (B) or RCA120 (C) as described in Materials and Methods. Lane 1 contains glycolipid standards Lc2Cer (2), Gb4Cer (4) and Gb5Cer (5). The arrowheads indicate the positions of IGLad.

    Journal:

    Article Title: Identification of an Intestinal Neutral Glycosphingolipid as a Phenotype-Specific Receptor for the K88ad Fimbrial Adhesin of Escherichia coli †

    doi:

    Figure Lengend Snippet: Binding of K88ad adhesin to intestinal neutral glycosphingolipids after β-galactosidase treatment. Neutral glycosphingolipids from a phenotype A animal were treated with β-galactosidase as described in Materials and Methods. Both treated (lane 3) and untreated (lane 2) glycosphingolipids (100 μg) were separated on HPTLC plates as described in Materials and Methods. Chromatograms were stained with orcinol-sulfuric acid reagent (A), or incubated with biotinylated K88ad adhesin (B) or RCA120 (C) as described in Materials and Methods. Lane 1 contains glycolipid standards Lc2Cer (2), Gb4Cer (4) and Gb5Cer (5). The arrowheads indicate the positions of IGLad.

    Article Snippet: Biotinylated vegetable lectins (concanavalin A lectin [ConA], soybean agglutinin [SBA], peanut agglutinin [PNA], Sambucus nigra agglutinin [SNA], Bandeira simplicifolia lectin [BSL II], Datura stramonium lectin [DSL], Jacalin, wheat germ agglutinin [WGA], Ulex europaeus agglutinin [UEA], Ricinus communis agglutinin [RCA 120 ], Maackia amurensis lectin I and II [MAL I and MAL II], Erythrina cristagalli lectin [ECL], Vicia villosa agglutinin [VVA]) were obtained from Vector Laboratories, Inc. (Burlingame, Calif.).

    Techniques: Binding Assay, High Performance Thin Layer Chromatography, Staining, Incubation

    Binding of K88ad adhesin to intestinal neutral glycosphingolipids after α-fucosidase treatment. Neutral glycosphingolipids from a phenotype A animal were treated with α-fucosidase as described in Materials and Methods. Both treated (lane 3) and untreated (lane 2) glycosphingolipids (100 μg) were separated on HPTLC plates as described in Materials and Methods. Chromatograms were stained with orcinol-sulfuric acid reagent (A) or incubated with biotinylated K88ad adhesin (B) or UEA (C) as described in Materials and Methods. Lane 1 contains glycolipid standards Lc2Cer (2), Gb4Cer (4) and Gb5Cer (5). The arrowheads indicate the positions of IGLad.

    Journal:

    Article Title: Identification of an Intestinal Neutral Glycosphingolipid as a Phenotype-Specific Receptor for the K88ad Fimbrial Adhesin of Escherichia coli †

    doi:

    Figure Lengend Snippet: Binding of K88ad adhesin to intestinal neutral glycosphingolipids after α-fucosidase treatment. Neutral glycosphingolipids from a phenotype A animal were treated with α-fucosidase as described in Materials and Methods. Both treated (lane 3) and untreated (lane 2) glycosphingolipids (100 μg) were separated on HPTLC plates as described in Materials and Methods. Chromatograms were stained with orcinol-sulfuric acid reagent (A) or incubated with biotinylated K88ad adhesin (B) or UEA (C) as described in Materials and Methods. Lane 1 contains glycolipid standards Lc2Cer (2), Gb4Cer (4) and Gb5Cer (5). The arrowheads indicate the positions of IGLad.

    Article Snippet: Biotinylated vegetable lectins (concanavalin A lectin [ConA], soybean agglutinin [SBA], peanut agglutinin [PNA], Sambucus nigra agglutinin [SNA], Bandeira simplicifolia lectin [BSL II], Datura stramonium lectin [DSL], Jacalin, wheat germ agglutinin [WGA], Ulex europaeus agglutinin [UEA], Ricinus communis agglutinin [RCA 120 ], Maackia amurensis lectin I and II [MAL I and MAL II], Erythrina cristagalli lectin [ECL], Vicia villosa agglutinin [VVA]) were obtained from Vector Laboratories, Inc. (Burlingame, Calif.).

    Techniques: Binding Assay, High Performance Thin Layer Chromatography, Staining, Incubation

    Binding of K88ad adhesin to purified neutral glycosphingolipids. Two micrograms of Lc3Cer (lane 1), Lc3Cer plus Lc4Cer (lane 2), nLc4Cer (lane 3), III4FucLc4Cer or Lea (lane 4), III3FucnLc4Cer or Lex (lane 5), V3FucnLc6Cer (lane 6), and VI2FucnLc6Cer (lane 7) were separated on HPTLC. These glycosphingolipids were either stained with orcinol-sulfuric acid reagent (A) or incubated with biotinylated K88ad adhesin (B) as described in Materials and Methods.

    Journal:

    Article Title: Identification of an Intestinal Neutral Glycosphingolipid as a Phenotype-Specific Receptor for the K88ad Fimbrial Adhesin of Escherichia coli †

    doi:

    Figure Lengend Snippet: Binding of K88ad adhesin to purified neutral glycosphingolipids. Two micrograms of Lc3Cer (lane 1), Lc3Cer plus Lc4Cer (lane 2), nLc4Cer (lane 3), III4FucLc4Cer or Lea (lane 4), III3FucnLc4Cer or Lex (lane 5), V3FucnLc6Cer (lane 6), and VI2FucnLc6Cer (lane 7) were separated on HPTLC. These glycosphingolipids were either stained with orcinol-sulfuric acid reagent (A) or incubated with biotinylated K88ad adhesin (B) as described in Materials and Methods.

    Article Snippet: Biotinylated vegetable lectins (concanavalin A lectin [ConA], soybean agglutinin [SBA], peanut agglutinin [PNA], Sambucus nigra agglutinin [SNA], Bandeira simplicifolia lectin [BSL II], Datura stramonium lectin [DSL], Jacalin, wheat germ agglutinin [WGA], Ulex europaeus agglutinin [UEA], Ricinus communis agglutinin [RCA 120 ], Maackia amurensis lectin I and II [MAL I and MAL II], Erythrina cristagalli lectin [ECL], Vicia villosa agglutinin [VVA]) were obtained from Vector Laboratories, Inc. (Burlingame, Calif.).

    Techniques: Binding Assay, Purification, High Performance Thin Layer Chromatography, Staining, Incubation

    Comigration of IGLad and nLc4Cer on HPTLC plates. Neutral glycosphingolipids from a phenotype A animal (100 μg, lane 2), and nLc4Cer (2 μg, lane 3) were separated on HPTLC. These glycosphingolipids were either stained with orcinol-sulfuric acid reagent (A) or incubated with biotinylated K88ad adhesin (B) as described in Materials and Methods. Lane 1 contains glycolipid standards (Lc2Cer [2], Gb4Cer [4], and Gb5Cer [5]). The arrowheads indicate the position of IGLad.

    Journal:

    Article Title: Identification of an Intestinal Neutral Glycosphingolipid as a Phenotype-Specific Receptor for the K88ad Fimbrial Adhesin of Escherichia coli †

    doi:

    Figure Lengend Snippet: Comigration of IGLad and nLc4Cer on HPTLC plates. Neutral glycosphingolipids from a phenotype A animal (100 μg, lane 2), and nLc4Cer (2 μg, lane 3) were separated on HPTLC. These glycosphingolipids were either stained with orcinol-sulfuric acid reagent (A) or incubated with biotinylated K88ad adhesin (B) as described in Materials and Methods. Lane 1 contains glycolipid standards (Lc2Cer [2], Gb4Cer [4], and Gb5Cer [5]). The arrowheads indicate the position of IGLad.

    Article Snippet: Biotinylated vegetable lectins (concanavalin A lectin [ConA], soybean agglutinin [SBA], peanut agglutinin [PNA], Sambucus nigra agglutinin [SNA], Bandeira simplicifolia lectin [BSL II], Datura stramonium lectin [DSL], Jacalin, wheat germ agglutinin [WGA], Ulex europaeus agglutinin [UEA], Ricinus communis agglutinin [RCA 120 ], Maackia amurensis lectin I and II [MAL I and MAL II], Erythrina cristagalli lectin [ECL], Vicia villosa agglutinin [VVA]) were obtained from Vector Laboratories, Inc. (Burlingame, Calif.).

    Techniques: High Performance Thin Layer Chromatography, Staining, Incubation